Moroni Rebecca

e-mail: rebecca.moroni@phd.unipi.it e rebe.moroni@gmail.com

Thesis supervisor: Prof. Francesco Camillo

Project Title: Ozonetherapy as a treatment for endometritis in Equidae

Abstract: Endometritis is an inflammation, with or without an infectious process, affecting the inner layer of the uterine mucosa; its persistence can make the uterine environment unfavorable to the conceptus, thus reducing the reproductive potential of the mare. Generally, it is treated through the use of uterine lavages, ecbolic and anti-inflammatory drugs, and antimicrobials, as and when needed. However, the lack of response to traditional therapy and the increasing incidence of antimicrobial-resistant pathogens have led to the development of alternative therapies for the treatment of mares with chronic endometritis, including platelet-rich plasma and ozone therapy (O3). Intrauterine treatment with O3 has been shown to be effective in reducing uterine inflammation in cows and in promoting local endometrial angiogenesis in mares. Furthermore, O3 therapy exhibits an antimicrobial effect, demonstrated both in vitro and in vivo. Therefore, since no studies are yet available that indicate a standardized protocol to be followed for the ozone treatment of endometritis in mares, our study will aim to evaluate the efficacy of intrauterine treatment based on a mixture of O2-O3 gas to treat this pathology.

Project title: Ozonetherapy as a treatment for endometritis in Equidae

Abstract: Endometritis is an inflammation, with or without an infectious process, affecting the inner layer of the uterine mucosa; its persistence can make the uterine environment unfavorable to the conceptus, thus reducing the reproductive potential of the mare. Generally, it is treated through the use of uterine lavages, ecbolic and anti-inflammatory drugs, and antimicrobials, as and when needed. However, the lack of response to traditional therapy and the increasing incidence of antimicrobial-resistant pathogens have led to the development of alternative therapies for the treatment of mares with chronic endometritis, including platelet-rich plasma and ozone therapy (O3). Intrauterine treatment with O3 has been shown to be effective in reducing uterine inflammation in cows and in promoting local endometrial angiogenesis in mares. Furthermore, O3 therapy exhibits an antimicrobial effect, demonstrated both in vitro and in vivo. Therefore, since no studies are yet available that indicate a standardized protocol to be followed for the ozone treatment of endometritis in mares, our study will aim to evaluate the efficacy of intrauterine treatment based on a mixture of O2-O3 gas to treat this pathology.

SCOPUS: https://www.scopus.com/authid/detail.uri?authorId=57220046535

ORCID ID: https://orcid.org/0000-0002-2108-1749

Publications:

  • Panzani D, Quaresma M, Fanelli D, Camillo F, Moroni R, Rota A, Martins-Bessa A, Nóvoa M, Catalán J, Canisso IF, Conte G, Mirò J. Hastening Time to Ejaculation in Donkey Jacks Treated with the PGF2α Analog, Cloprostenol Sodium. Animals. 2020;10(12):2231. https://doi.org/10.3390/ani10122231
  • Fanelli D, Moroni R, Bocci C, Camillo F, Rota A, Panzani D. Interspecific and Intraspecific Artificial Insemination in Domestic Equids. Animals. 2023;13(4):582. https://doi.org/10.3390/ani13040582
  • Moroni R, Panzani D, Camillo F, Rota A, Fanelli D, Cantile C, Marmorini P, Salamone G, Ori M. Endometrial expression of antimicrobial peptides as markers of subclinical endometritis in mares. Equine veterinary journal. 2024; 56(4):670–677. https://doi.org/10.1111/evj.14080
  • Martini M, Degl’Innocenti A, Altomonte I, Sodi I, Bocci C, Fanelli D, Moroni R, Panzani D, Camillo F, Salari F. Report on a Milking Mule: Milk Qualitative Characteristics during Lactation. Animals. 2024; 14(11):1585. https://doi.org/10.3390/ani14111585

Scopus link: https://www.scopus.com/authid/detail.uri?authorId=57220046535

ORCID ID: https://orcid.org/0000-0002-2108-1749

Oral communications at conferences:

  • Moroni*, D. Fanelli, M. Tesi, B. Cerretini, D. Lomet, A. Rota, M. Beltramo, F. Camillo, D. Panzani. Effect of administration of the C6 kisspeptin analogue in jennies in estrous. 10th ISEET, Pisa, 2022.
  • Moroni R*, Marmorini P, Fanelli D, Melanie P, Cantile C, Passamonti F, Camillo F, Rota A, Panzani D. The effects of uterine ozone insufflation in mares affected by endometritis: preliminary results. 76° convegno SISVET, Bari, 2023.
  • Moroni R*, Ori M, Camillo F, Rota A, Fanelli D, Cantile C, Marmorini P, Salamone G, Panzani D. Endometrial expression of antimicrobial peptides as markers of subclinical endometritis in mares. 1st Game of Research, Pisa, 2023.
  • Moroni R*, Maltinti S, Fanelli D, Camillo F, Rota A, Cantile C, Marmorini P, Orefice M, Ori M, Panzani D. Endometrial expression of antimicrobial peptides as markers of subclinical endometritis in mares. 2nd ECAR, Zurigo, 2024.

Poster at conferences:

  • Bocci*, D. Fanelli, R. Moroni, M. Tesi, F. Camillo, S. Wilsher, W.R. Allen. Pregnancy rates in equids after interspecific and intraspecific artificial insemination. 10th ISEET, Pisa, 2022.
  • Fanelli*, L. Losinno, C. Castañeira, C. Alonso, A. Flores Bragulat, D. Panzani, C. Bocci, A. Degl’Innocenti,R. Moroni, F. Camillo, S. Wilsher, W.R. Allen. First report of mule-in-mule pregnancies with live births following embryo transfer. 10th ISEET, Pisa, 2022.
  • Moroni R*, Panzani D, Camillo F, Rota A, Fanelli D, Franco AM, Ori Endometrial gene expression of DEFB4B, LYZ, and SLPI in biopsy samples of mares resistant or susceptible to endometritis. 13° ISER, Brasile, 2023.
  • Fanelli D*, Moroni R, Camillo F, Rota A, Panzani D. The GnRH analogue Decapeptyl was able to induce ovulation in cyclic jennies. 13° ISER, Brasile, 2023.
  • Fanelli, L. Losinno, C. Castañeira, C. Alonso, A. Flores Bragulat, D. Panzani, C. Bocci, A. Degl’Innocenti, R. Moroni*, F. Camillo, S. Wilsher, W.R. Allen. First report of mule-in-mule pregnancies with live births following embryo transfer. 1st Game of Research, Pisa, 2023.